Immunofluorescence of human HeLa cells stained with Hoechst 3342 (Blue) for nucleus staining and monoclonal anti-human LDHA antibody (1:500) with Texas Red (Red).

Cell lysates of HeLa (35ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human LDHA (1:8000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.