Rat tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with LGI1 antibody [N2C2], Internal (GTX105700) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

LGI1 antibody [N2C2], Internal detects LGI1 protein by immunofluorescent analysis.
Sample: DIV10 rat E18 primary hippocampal neurons were fixed in 4% paraformaldehyde at RT for 15 min.
Green: LGI1 protein stained by LGI1 antibody [N2C2], Internal (GTX105700) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with LGI1 antibody [N2C2], Internal (GTX105700) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Sample (30 ug of whole cell lysate)
A: A431 (GTX27909)
7.5% SDS PAGE
GTX105700 diluted at 1:5000