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LGR6 antibody [HL2827]

Recombinant antibody Orthogonal
Anti-LGR6 antibody [HL2827] used in IHC (Paraffin sections) (IHC-P). GTX640098
Anti-LGR6 antibody [HL2827] used in IHC (Paraffin sections) (IHC-P). GTX640098
Anti-LGR6 antibody [HL2827] used in Western Blot (WB). GTX640098

Cat. No. GTX640098

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL2827

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all LGR6 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:5000-1:100000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

104 kDa. ( Note )

Product Note

This antibody was raised against human LGR6 Intracellular domain.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the Intracellular domain of human LGR6. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

leucine rich repeat containing G protein-coupled receptor 6 , GPCR , VTS20631

Cellular Localization

Cell membrane

Background

This gene encodes a member of the leucine-rich repeat-containing subgroup of the G protein-coupled 7-transmembrane protein superfamily. The encoded protein is a glycoprotein hormone receptor with a large N-terminal extracellular domain that contains leucine-rich repeats important for the formation of a horseshoe-shaped interaction motif for ligand binding. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-LGR6 antibody [HL2827] used in IHC (Paraffin sections) (IHC-P). GTX640098

GTX640098 IHC-P Image

LGR6 antibody [HL2827] detects LGR6 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded human A549 xenograft.
LGR6 stained by LGR6 antibody [HL2827] (GTX640098) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 3 min

Anti-LGR6 antibody [HL2827] used in IHC (Paraffin sections) (IHC-P). GTX640098

GTX640098 IHC-P Image

LGR6 antibody [HL2827] detects LGR6 protein by immunohistochemical analysis.
Sample: Paraffin-embedded human colon cancer.
LGR6 stained by LGR6 antibody [HL2827] (GTX640098) diluted at 1:100.
Antigen Retrieval: Tris-EDTA buffer, pH 8.0, 15 min

Anti-LGR6 antibody [HL2827] used in Western Blot (WB). GTX640098

GTX640098 WB Image

Orthogonal

Non-transfected (–) and transfected (+) boiled and unboiled 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with LGR6 antibody [HL2827] (GTX640098) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations