APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:500-1:3000 |
1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
MCF-7 , MDA-MB-231
Predict Reactivity
Mouse, Rat, Zebrafish, Bovine(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human LIN7A. The exact sequence is proprietary.
Purification
Affinity purified by Protein G.
Conjugation
Unconjugated
RRID
AB_2888239
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
lin-7 homolog A, crumbs cell polarity complex component , LIN-7A , LIN7 , MALS-1 , MALS1 , TIP-33 , VELI1
Cellular Localization
Cell membrane; Peripheral membrane protein , Basolateral cell membrane; Peripheral membrane protein , Cell junction , Cell junction , synapse , postsynaptic cell membrane , postsynaptic density; Peripheral membrane protein
Background
Plays a role in establishing and maintaining the asymmetric distribution of channels and receptors at the plasma membrane of polarized cells. Forms membrane-associated multiprotein complexes that may regulate delivery and recycling of proteins to the correct membrane domains. The tripartite complex composed of LIN7 (LIN7A, LIN7B or LIN7C), CASK and APBA1 may have the potential to couple synaptic vesicle exocytosis to cell adhesion in brain. Ensures the proper localization of GRIN2B (subunit 2B of the NMDA receptor) to neuronal postsynaptic density and may function in localizing synaptic vesicles at synapses where it is recruited by beta-catenin and cadherin. Required to localize Kir2 channels, GABA transporter (SLC6A12) and EGFR/ERBB1, ERBB2, ERBB3 and ERBB4 to the basolateral membrane of epithelial cells.
Database
Research Area
DATA IMAGES
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GTX631106 ICC/IF Image
LIN7A antibody [GT377] detects LIN7A protein at junction by immunofluorescent analysis. Sample: MCF-7 cells were fixed in ice-cold MeOH for 5 min. Green: LIN7A protein stained by LIN7A antibody [GT377] (GTX631106) diluted at 1:1000. Blue: Hoechst 33342 staining.
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GTX631106 WB Image
LIN7A antibody [GT377] detects LIN7A protein by western blot analysis. A. 30 μg MCF-7 whole cell extract B. 30 μgMDA-MB-231 whole cell extract 12 % SDS-PAGE LIN7A antibody [GT377] (GTX631106) dilution: 1:1000
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REFERENCE
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REVIEW
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