Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with LRP130 antibody [N3C2], Internal (GTX109558) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Sample (30 μg of whole cell lysate)
A: H1299
B: HeLa
5% SDS PAGE
GTX109558 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

LRP130 antibody [N3C2], Internal detects LRP130 protein at cytoplasm in human colon cancer by immunohistochemical analysis.
Sample: Paraffin-embedded human colon cancer.
LRP130 antibody [N3C2], Internal (GTX109558) diluted at 1:100.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

LRP130 antibody [N3C2], Internal detects LRP130 protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: LRP130 protein stained by LRP130 antibody [N3C2], Internal (GTX109558) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Immunohistochemical analysis of paraffin-embedded SW480 Xenograft, using LRP130 (GTX109558) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Various tissue extracts (50 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with LRP130 antibody [N3C2], Internal (GTX109558) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.