Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with LRP6 (phospho Ser1490) antibody (GTX130421) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with LRP6 phospho S1490 antibody (GTX130421) diluted at 1:1000. (CIP: Calf-intestinal alkaline phosphatase)