RAP antibody [N1C3] detects RAP protein at cytoplasm in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
RAP antibody [N1C3] (GTX104033) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

RAP antibody [N1C3] detects RAP protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: RAP protein stained by RAP antibody [N1C3] (GTX104033) diluted at 1:200.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with RAP antibody [N1C3] (GTX104033) diluted at 1:1000.

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with RAP antibody [N1C3] (GTX104033) diluted at 1:1000.

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with RAP antibody [N1C3] (GTX104033) diluted at 1:1000.