Wild-type (WT) and LXN knockout (KO) HeLa cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with LXN antibody [N1C3] (GTX107116) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunohistochemical analysis of paraffin-embedded FaDu xenograft, using LXN(GTX107116) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Sample (50 μg of whole cell lysate)
A: mouse brain
12% SDS PAGE
GTX107116 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunofluorescence analysis of paraformaldehyde-fixed mouse ESC D3, using LXN(GTX107116) antibody at 1:200 dilution.

Rat tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with LXN antibody [N1C3] (GTX107116) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Sample(30 μg whole cell lysate)
A:293T
B:HeLa S3(GTX14654)
12% SDS PAGE
GTX107116 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Rat tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with LXN antibody [N1C3] (GTX107116) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.