Wild-type (WT) and CD44 knockout (KO) HeLa cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with LYN antibody (GTX111584) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

LYN antibody detects LYN protein by Western blot analysis.
A. 30 μg THP-1 whole cell lysate/extract
7.5 % SDS-PAGE
LYN antibody (GTX111584) dilution: 1:1000

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed A431, using LYN(GTX111584) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

LYN antibody immunoprecipitates LYN protein in IP experiments.
IP samples: K562 whole cell extract
A. 40 μg K562 whole cell extract
B. Control with 4 μg of preimmune Rabbit IgG
C. Immunoprecipitation of LYN protein by 4 μg LYN antibody (GTX111584)
5 % SDS-PAGE
The immunoprecipitated LYN protein was detected by LYN antibody (GTX111584) diluted at 1:500.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]