ICC/IF analysis of HeLa cells using GTX80813 Lamin A + C antibody [mab636].

ICC/IF analysis of A549 cells using GTX80813 Lamin A + C antibody [mab636].
Green : Primary antibody
Blue : Nuclei

ICC/IF analysis of MDCK cells using GTX80813 Lamin A + C antibody [mab636].
Red : Primary antibody
Blue : cell nucleus
Permeabilization : 0.1% Triton X-100
Dilution : 1:100 in blocking buffer for at least 1 hour at room temperature

ICC/IF analysis of U2-OS cells (A) or HeLa cells (B) using GTX80813 Lamin A + C antibody [mab636].
Green : Primary antibody
Blue : Nuclei
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 10 minutes
Dilution : 1:100 for at least 1 hour at room temperature

WB analysis of various amounts of human fibroblast whole cell lysate using GTX80813 Lamin A + C antibody [mab636].
Dilution : 1:2000

WB analysis of 10μg nuclear extract from fractionated mouse tissue lysate using GTX80813 Lamin A + C antibody [mab636].
Dilution : 1:500

ICC/IF analysis of HMVEC Cells using GTX80813 Lamin A + C antibody [mab636].
Green : Primary antibody
Blue : Nuclei

ICC/IF analysis of U251 cells using GTX80813 Lamin A + C antibody [mab636]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:200 overnight at 4ºC

ICC/IF analysis of Human fibroblast cells using GTX80813 Lamin A + C antibody [mab636].
Fixation : methanol
Permeabilization : 0.1% Triton X-100 in TBS for 10 minutes
Dilution : 1:200 for at least 1 hour at room temperature