Summary
Laminins, which consist of three subunits called alpha, beta and gamma chains, are major cell-adhesive components of extracellular matrix, especially basement membranes (BMs). The laminin family is constituted of over 15 isoforms, and each member is expressed in a tissue-specific manner and plays a differential role in each tissue. Laminin-511 (Lm511) (formerly laminin-10), which consists of alpha 5, beta 1 and gamma 1 chains, appears at an early stage of development and is most widely expressed in adult tissues, mainly basement membranes of various epithelial tissues and vasculatures. Recently, laminin-511 is often used as a culture substrate for ES and iPS cells.This antibody specifically interacts with the trimeric structure of laminin-511 isoform but does not react with any of the alpha 5, beta 1 and gamma 1 chains in the reducing conditions. Furthermore, it has been validated that clone 12D does not react with any of laminins-111, -211, -332, -3B32, -411 and -521. Unlike conventional anti- alpha 5 antibodies, clone 12D distinguishes laminin-511 from laminin-521. This is an important tool to evaluate specific distribution and physiological functions of laminin-511. In human cancers, clone 12D detects small vascular vessels with high sensitivity and hence useful for evaluating tumor angiogenesis.
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:1000 |
Assay dependent |
Assay dependent |
Assay dependent |
Assay dependent |
Not tested in other applications.
Product Note
This antibody specifically interacts with the trimeric structure of laminin-511 isoform but does not react with any of the alpha 5, beta 1 and gamma 1 chains in the reducing conditions. Furthermore, it has been validated that clone 12D does not react with any of laminins-111, -211, -332, -3B32, -411 and -521. Unlike conventional anti- alpha 5 antibodies, clone 12D distinguishes laminin-511 from laminin-521.
Form
Liquid
Buffer
Ascites
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Antigen Species
Human
Immunogen
Human placental laminin.
Purification
Unpurified
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.