Laminin 511 antibody [12D]

Cat. No. GTX17688
References 1
Cat. No.  GTX17688
Anti-Laminin 511 antibody [12D] used in Western Blot (WB). GTX17688
GTX17688 WB Image

WB analysis of recombinant human intact laminin-511 (Lm511, containing consisting of alpha5, beta 1 and gamma 1 chains ) protein under non-reducing conditions using GTX17688 Laminin 511 antibody [12D].
Dilution : 1:1000

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Anti-Laminin 511 antibody [12D] used in IHC (Frozen sections) (IHC-Fr). GTX17688
GTX17688 IHC-Fr Image

IHC-Fr analysis of normal mammary gland using GTX17688 Laminin 511 antibody [12D].
Black arrows : Vascular basement membrane
White arrowheads : Mammary gland basement membrane

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Anti-Laminin 511 antibody [12D] used in IHC (Frozen sections) (IHC-Fr). GTX17688
GTX17688 IHC-Fr Image

IHC-Fr analysis of lung cancer (SCC and ADC) tissue using GTX17688 Laminin 511 antibody [12D].
Left panel : Lung squamous cell carcinoma (SCC)
Right panel : lung adenocarcinoma (ADC)

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Anti-Laminin 511 antibody [12D] used in (). GTX17688
GTX17688 Image

Protein structure of laminin-511

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Anti-Laminin 511 antibody [12D] used in ELISA (ELISA). GTX17688
GTX17688 ELISA Image

ELISA anaiysis of seven recombinant human laminins using GTX17688 Laminin 511 antibody [12D].

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Anti-Laminin 511 antibody [12D] used in IHC (Frozen sections) (IHC-Fr). GTX17688
GTX17688 IHC-Fr Image

IHC-Fr analysis of non-invasive breast carcinoma tissue using GTX17688 Laminin 511 antibody [12D].
Image A : Vascular basement membrane
Image B : Mammary gland basement membrane
Image C : Basement membrane-like structures surrounding tumor cells

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Anti-Laminin 511 antibody [12D] used in Western Blot (WB). GTX17688
Anti-Laminin 511 antibody [12D] used in IHC (Frozen sections) (IHC-Fr). GTX17688
Anti-Laminin 511 antibody [12D] used in IHC (Frozen sections) (IHC-Fr). GTX17688
Anti-Laminin 511 antibody [12D] used in (). GTX17688
Anti-Laminin 511 antibody [12D] used in ELISA (ELISA). GTX17688
Anti-Laminin 511 antibody [12D] used in IHC (Frozen sections) (IHC-Fr). GTX17688
  • Host
    Mouse
  • Clonality
    Monoclonal
  • Clone Name
    12D
  • Isotype
    IgG2b
  • Applications
    WB IHC-Fr IP ELISA Purification
  • Reactivity
    Human
Summary
Laminins, which consist of three subunits called alpha, beta and gamma chains, are major cell-adhesive components of extracellular matrix, especially basement membranes (BMs). The laminin family is constituted of over 15 isoforms, and each member is expressed in a tissue-specific manner and plays a differential role in each tissue. Laminin-511 (Lm511) (formerly laminin-10), which consists of alpha 5, beta 1 and gamma 1 chains, appears at an early stage of development and is most widely expressed in adult tissues, mainly basement membranes of various epithelial tissues and vasculatures. Recently, laminin-511 is often used as a culture substrate for ES and iPS cells.This antibody specifically interacts with the trimeric structure of laminin-511 isoform but does not react with any of the alpha 5, beta 1 and gamma 1 chains in the reducing conditions. Furthermore, it has been validated that clone 12D does not react with any of laminins-111, -211, -332, -3B32, -411 and -521. Unlike conventional anti- alpha 5 antibodies, clone 12D distinguishes laminin-511 from laminin-521. This is an important tool to evaluate specific distribution and physiological functions of laminin-511. In human cancers, clone 12D detects small vascular vessels with high sensitivity and hence useful for evaluating tumor angiogenesis.
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