Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with Laminin beta 3 antibody [C1C2], Internal (GTX103736) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Laminin beta 3 antibody [C1C2], Internal detects Laminin beta 3 protein by immunofluorescent analysis.
Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Laminin beta 3 stained by Laminin beta 3 antibody [C1C2], Internal (GTX103736) diluted at 1:500.
Blue: Hoechst 33342 staining.

Sample (30 μg of whole cell lysate)
A:NIH-3T3
7.5% SDS PAGE
GTX103736 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunohistochemical analysis of paraffin-embedded human lung cancer, using Laminin beta 3(GTX103736) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min