Name: Laminin gamma 2 antibody [P2H]
Brand: GeneTex
SKU: GTX17686
Price: 419.00 USD

Laminin gamma 2 antibody [P2H]

Cat. No. GTX17686

GTX17686 IHC-P Image

IHC-P analysis of lung cancer (SCC) tissue using GTX17685 Laminin alpha 3A antibody [BG5] and GTX17686 Laminin gamma 2 chain (N-terminal region) antibody [P2H]. The GTX17685 detected basement membrane (BM)-like structure surrounding tumor cells. GTX17686 detected not only BMs but also the cytoplasm of cancer cells.
Upper panel : GTX17686
Lower panel : GTX17685

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GTX17686 Image

Protein structure of laminin-3A32s and cleavage site/N-terminal fragment of gamma 2 chain

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GTX17686 IHC-P Image

IHC-P analysis of normal skin and skin cancer using GTX17686 Laminin gamma 2 chain (N-terminal region) antibody [P2H]. The GTX17686 stains epithelial basement membranes in normal skin, in a skin cancer tissue it strongly stains invading cancer cells or their cytoplasm.
Upper panel : Normal skin tissue
Lower panel : Skin cancer tissue

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GTX17686 IHC-P Image

IHC-P analysis of normal mammary gland and breast cancer using GTX17686 Laminin gamma 2 chain (N-terminal region) antibody [P2H]. The GTX17686 stains basement membranes surrounding mammary glands in normal tissue, whereas in a breast cancer tissue cytoplasmic accumulation of gamma 2pf or its fragments is detected with P2H.
Upper panel : Normal mammary gland
Lower panel : Breast cancer

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GTX17686 IHC-P Image

IHC-P analysis of invasive lung cancer (SCC and ADC) tissue using GTX17686 Laminin gamma 2 chain (N-terminal region) antibody [P2H]. The GTX17686 strongly detected cytoplasm of cancer cells at invasion fronts.
Upper panel : SCC
Lower panel : ADC

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GTX17686 WB Image

WB analysis of various samples using GTX17686 Laminin gamma 2 chain (N-terminal region) antibody [P2H]. Small gamma 2 N-terminal fragments are seen below the gamma 2pf band (45 kDa), and 15-kDa bands corresponds to NE1-2 and NE2-3.
Left : recombinant human gamma 2pf under reducing conditions, 10% gel
Right : conditioned medium of VMRC-LCP lung cancer cells, gradient gel
Dilution : 1:1000

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Host

Mouse
Clonality

Monoclonal
Clone Name

P2H
Isotype

IgG2a
Applications

WB IHC-P ELISA IHC
Reactivity

Human, Mouse

Summary

Laminins, which consist of three subunits called alpha, beta and gamma chains, are major cell-adhesive components of extracellular matrix, especially basement membranes (BMs). The laminin family is constituted of over 15 isoforms, and each member is expressed in a tissue-specific manner and plays a differential role in each tissue. Laminin gamma 2 chain is a subunit of laminin-332 isoform, formerly laminin-5, but it is frequently overexpressed as a monomer form or the beta3-gamma2 heterodimer in invasive cancers. Laminin gamma 2 is a protein of approximately 150 kDa and cleaved at its short arm mainly by bone morphogenic protein-1 (BMP1) in cancer cells, releasing an N-terminal proteolytic fragment (gamma 2pf) of 45 kDa. The short arm and gamma 2pf are also cleaved by MMPs and serine proteinases, releasing further small N-terminal fragments.This antibody recognizes laminin gamma 2 chain of laminin-332 on normal epithelial basement membranes, while in malignant cancer tissues it strongly immunostains the cytoplasm of cancer cells at invasion fronts. Thus, P2H is an important antibody capable of highly detecting the tumor invasion marker laminin gamma 2 chain. P2H is an only commercially available antibody to detect the N-terminal proteolytic fragments of laminin gamma 2 chain in cancer tissues. It is useful for pathological assessment of invasiveness and malignancy in various types of human cancers.