Various tissue extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Lck antibody [N3C3] (GTX107785) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Sample (30 ug of whole cell lysate)
A: 293T
B: Molt-4 (GTX27912)
7.5% SDS PAGE
GTX107785 diluted at 1:1000