MCF-7 whole cell and membrane extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Leptin antibody (GTX109204) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Leptin antibody (GTX109204) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Leptin antibody detects Leptin protein by Western blot analysis.
A. 30 μg MCF-7 whole cell lysate/extract
B. 30 μg MDA-MB-231 whole cell lysate/extract
12 % SDS-PAGE
Leptin antibody (GTX109204) dilution: 1:1000

Leptin antibody detects Leptin protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse adrenal gland.
Leptin stained by Leptin antibody (GTX109204) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Leptin antibody detects Leptin protein at secreted on human lung papillary adenocarcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human lung papillary adenocarcinoma.
Leptin antibody (GTX109204) diluted at 1:250.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min