MAD1 antibody detects MAD1L1 protein by western blot analysis.
A. 30 μg Neuro2A whole cell lysate/extract
B. 30 μg GL261 whole cell lysate/extract
C. 30 μg C8D30 whole cell lysate/extract
D. 30 μg NIH-3T3 whole cell lysate/extract
E. 30 μg BCL-1 whole cell lysate/extract
F. 30 μg Raw264.7 whole cell lysate/extract
7.5% SDS-PAGE
MAD1 antibody (GTX109519) dilution: 1:3000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunohistochemical analysis of paraffin-embedded SW480 Xenograft, using Mad 1 (GTX109519) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Immunoprecipitation of MAD1 protein from HeLa whole cell extracts using 5 μg of MAD1 antibody (GTX109519).
Western blot analysis was performed using MAD1 antibody (GTX109519).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

MAD1 antibody detects MAD1 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: MAD1 protein stained by MAD1 antibody (GTX109519) diluted at 1:500.
Red: HEC1, a kinetochore marker, stained by HEC1 antibody [9G3.23] (GTX70268) diluted at 1:500.
Blue: Hoechst 33342 staining.

MAD1 antibody detects MAD1L1 protein by western blot analysis.
A. 30 μg PC-12 whole cell lysate/extract
B. 30 μg Rat2 whole cell lysate/extract
7.5% SDS-PAGE
MAD1 antibody (GTX109519) dilution: 1:3000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

MAD1 antibody detects MAD1 protein by Western blot analysis. Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MAD1 antibody (GTX109519) diluted by 1:5000.

Sample (30 μg of whole cell lysate)
A: Molt-4 (GTX27912)
7.5% SDS PAGE
GTX109519 diluted at 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

The data was published in the journal Nat Commun in 2015.PMID: 26620470

The data was published in the journal Nat Commun in 2015.PMID: 26620470

The data was published in the journal Nat Commun in 2016. PMID: 26743940