IHC-Fr analysis of mouse embryos using GTX00754 MAGED1 antibody.
The arrowheads indicate the ventricular proliferative zone; V : Ventricle; C : Bone Cavity. MAGE-D1 was concentrated in the preplate of the forebrain at E12.5 and skeletal muscle tissues in the hind limb at E14.5. In developing neural tube, MAGE-D1 immunoreactivity was distributed in the ventricular zone as well as the marginal zone.
Upper : E12.5
Lower : E14.5
Dilution : 1:300

WB analysis of the cerebral cortex from mouse embryo (E16.5) using GTX00754 MAGED1 antibody.
Dilution : 1:3000
Loading : 10 μg

WB analysis of various mouse embryo samples (E18.5) using GTX00754 MAGED1 antibody. Endogenous ~85-kDa MAGE-D1 protein was expressed in a ubiquitous manner.
Loading : 20 μg

ICC/IF analysis of C2C12 cells using GTX00754 MAGED1 antibody (D1) or MHC antibody (MHC). C2C12 myoblast cells were cultured under undifferentiated conditions (UD) or under differentiation conditions for 4 days (4D). The arrowheads point to differentiated multinucleated myocytes. MHC and MAGE-D1 were distributed predominantly in the cytosol of multinucleated differentiated C2C12 cells.