Wild-type (WT) and MAPKAP Kinase 3 knockout (KO) HeLa cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with MAPKAP Kinase 3 antibody [N2C3] (GTX105147) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Sample (50 ug of whole cell lysate)
A: mouse spleen
10% SDS PAGE
GTX105147 diluted at 1:10000

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using MAPKAP Kinase 3 (GTX105147) antibody at 1:200 dilution.

Sample(30 ug whole cell lysate)
A:H1299
B:HeLa S3(GTX14654)
10% SDS PAGE
GTX105147 diluted at 1:3000