Drosophila tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with MAT1A + MAT2A antibody [HL1679] (GTX637278) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with MAT1A + MAT2A antibody [HL1679] (GTX637278) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with MAT1A + MAT2A antibody [HL1679] (GTX637278) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with MAT1A + MAT2A antibody [HL1679] (GTX637278) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.