ELISA analysis of peptides used for immunization using GTX60359 MBD1 antibody - ChIP grade.

ChIP analysis of sheared chromatin from 10⁶ U2OS cells using GTX60359 MBD1 antibody - ChIP grade. Beads only were used as a negative IP control. Quantitative PCR was performed with primers for the promoters of the MLH1 gene (used as a positive control) and CDC6 gene (used as a negative control). This figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

WB analysis of nuclear extracts (40 μg) from HeLa cells using GTX60359 MBD1 antibody - ChIP grade.
Dilution : 1:500