Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with MC1 Receptor antibody [C2C3], C-term (GTX108190) diluted at 1:4000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

MC1 Receptor antibody [C2C3], C-term detects MC1R protein at cytosol and membrane on human colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded colon carcinoma.
MC1 Receptor antibody [C2C3], C-term (GTX108190) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

MC1 Receptor antibody [C2C3], C-term detects MC1R protein at membrane on mouse fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse fore brain.
MC1 Receptor antibody [C2C3], C-term (GTX108190) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Mouse tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with MC1 Receptor antibody [C2C3], C-term (GTX108190) diluted at 1:4000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.