Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with MCM3 antibody [N1N3] (GTX111160) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Sample (30 ug of whole cell lysate)
A: Hep G2 (GTX27900)
7.5% SDS PAGE
GTX111160 diluted at 1:10000

MCM3 antibody [N1N3] detects MCM3 protein at nucleus on mouse colon by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
MCM3 antibody [N1N3] (GTX111160) diluted at 1:1000.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MCM3 antibody [N1N3] (GTX111160) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using MCM3(GTX111160) antibody at 1:500 dilution.