PRODUCT
APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:100-1:200 |
Note :
IHC-P
Manual staining : Heat-induced antigen retrieval for 5 minutes in an autoclave at 121ºC in pH 7.8 Tris-EDTA-based Target Retrieval Solution buffer.
This antibody has been validated by Autostainer including Agilent/Dako - Autostainer Link 48, Bond RX-Lecia, and Roche-Ventana Discovery ULTRA. The detailed steps by Autostainer please refer to the protocol.
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Not tested in other applications.
Calculated MW
Positive Control
Colon: A strong nuclear MCM7 immunostaining should be seen in virtually all crypt base cells.
Negative Control
Colon: MCM7 immunostaining should be largely absent in surface epithelial cells and in most stroma cells.
PROPERTIES
Form
Liquid
Buffer
PBS, 0.05% BSA (Please contact us for PBS only format)
Preservative
0.05% sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant human MCM7 protein fragment (aa195-319)
Purification
Protein A/G purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
minichromosome maintenance complex component 7 , CDC47 , MCM2 , P1.1-MCM3 , P1CDC47 , P85MCM , PNAS146 , PPP1R104
Cellular Localization
Nucleus
Background
The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 4 and 6 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. Cyclin D1-dependent kinase, CDK4, is found to associate with this protein, and may regulate the binding of this protein with the tumorsuppressor protein RB1/RB. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
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GTX04444 IHC-P Image
IHC-P analysis of human lymph node tissue section using GTX04444 MCM7 antibody [MSVA-507R] HistoMAX™. In lymph nodes a particularly strong MCM7 staining occurs in most cells of germinal centres.
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GTX04444 IHC-P Image
IHC-P analysis of human colorectal adenocarcinoma tissue section using GTX04444 MCM7 antibody [MSVA-507R] HistoMAX™. Colorectal adenocarcinoma with a large fraction of MCM7 positive tumor cells.
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GTX04444 IHC-P Image
IHC-P analysis of human prostatic adenocarcinoma tissue section using GTX04444 MCM7 antibody [MSVA-507R] HistoMAX™. Prostatic adenocarcinoma (Gleason 336) with MCM7 staining of a limited number of tumor cells.
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REFERENCE
There are currently no references for MCM7 antibody [MSVA-507R] HistoMAX™ (GTX04444). Be the first to share your publications with this product.
REVIEW
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