Boiled and unboiled HeLa whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with MCT4 antibody (GTX131626) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Unboiled HeLa whole cell and membrane extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with MCT4 antibody (GTX131626) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. (WCE: whole cell extract; ME: membrane extract)