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MDM2 antibody

Orthogonal
Anti-MDM2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100531
Anti-MDM2 antibody used in Western Blot (WB). GTX100531
Anti-MDM2 antibody used in IHC (Paraffin sections) (IHC-P). GTX100531
Anti-MDM2 antibody used in Immunoprecipitation (IP). GTX100531
Anti-MDM2 antibody used in Western Blot (WB). GTX100531
Anti-MDM2 antibody used in IHC (Paraffin sections) (IHC-P). GTX100531
Anti-MDM2 antibody used in ChIP assay (ChIP assay). GTX100531
Anti-MDM2 antibody used in Western Blot (WB). GTX100531
Anti-MDM2 antibody used in IHC (Paraffin sections) (IHC-P). GTX100531

Cat. No. GTX100531

Reference 9
1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP, ChIP assay

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all MDM2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
ChIP assay Assay dependent
Not tested in other applications.

Calculated MW

55 kDa. ( Note )

Positive Control

HepG2 (starvation and 1 μM Staurosporine treatment for 24 hr)

Predict Reactivity

Rat, Bovine, Cat, Dog, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.68 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human MDM2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10624270

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

MDM2 proto-oncogene , ACTFS , HDMX , hdm2

Cellular Localization

Nucleus , nucleoplasm , Cytoplasm , nucleolus

Background

This gene is a target gene of the transcription factor tumor protein p53. The encoded protein is a nuclear phosphoprotein that binds and inhibits transactivation by tumor protein p53, as part of an autoregulatory negative feedback loop. Overexpression of this gene can result in excessive inactivation of tumor protein p53, diminishing its tumor suppressor function. This protein has E3 ubiquitin ligase activity, which targets tumor protein p53 for proteasomal degradation. This protein also affects the cell cycle, apoptosis, and tumorigenesis through interactions with other proteins, including retinoblastoma 1 and ribosomal protein L5. More than 40 different alternatively spliced transcript variants have been isolated from both tumor and normal tissues. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-MDM2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100531

GTX100531 ICC/IF Image

MDM2 antibody detects MDM2 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: MDM2 stained by MDM2 antibody (GTX100531) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Scale bar= 10μm.

Anti-MDM2 antibody used in Western Blot (WB). GTX100531

GTX100531 WB Image

Orthogonal

Untreated (–) and treated (+) HCT116 whole cell extract (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MDM2 antibody (GTX100531) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-MDM2 antibody used in IHC (Paraffin sections) (IHC-P). GTX100531

GTX100531 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human lung cancer, using MDM2(GTX100531) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-MDM2 antibody used in Immunoprecipitation (IP). GTX100531

GTX100531 IP Image

MDM2 antibody immunoprecipitates MDM2 protein in IP experiments.
IP samples: Jurkat whole cell extract
A. 30 μg Jurkat cell whole cell extract
B. Control with 4 μg of preimmune Rabbit IgG
C. Immunoprecipitation of MDM2 protein by 4 μg MDM2 antibody (GTX100531)
7.5 % SDS-PAGE
The immunoprecipitated MDM2 protein was detected by MDM2 antibody (GTX100531) diluted at 1:500.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
Anti-MDM2 antibody used in Western Blot (WB). GTX100531

GTX100531 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MDM2 antibody (GTX100531) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-MDM2 antibody used in IHC (Paraffin sections) (IHC-P). GTX100531

GTX100531 IHC-P Image

MDM2 antibody detects MDM2 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
MDM2 stained by MDM2 antibody (GTX100531) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-MDM2 antibody used in ChIP assay (ChIP assay). GTX100531

GTX100531 ChIP assay Image

Cross-linked ChIP was performed with PC-3 chromatin extract and 5 μg of either control rabbit IgG or anti-MDM2 antibody. The precipitated DNA was detected by PCR with primer set targeting to PSA promoter.
ChIP experiment and primer designs are based on Nucleic Acids Res. 2005 Jan 7;33(1):13-26.

Anti-MDM2 antibody used in Western Blot (WB). GTX100531

GTX100531 WB Image

Orthogonal

Untreated (–) and treated (+) HepG2 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MDM2 antibody (GTX100531) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-MDM2 antibody used in IHC (Paraffin sections) (IHC-P). GTX100531

GTX100531 IHC-P Image

MDM2 antibody detects MDM2 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
MDM2 stained by MDM2 antibody (GTX100531) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

HC Cheng et al. Cell Mol Life Sci 2023; 80 (11) : 338 Loss?of?fragile?WWOX?gene?leads?to?senescence?escape?and?genome?instability
Tateishi K et al. Acta Neuropathol Commun 2023; 11 (1) : 186 Genetic alterations that deregulate RB and PDGFRA signaling pathways drive tumor progression in IDH2-mutant astrocytoma.
Zeng M et al. Cancer Manag Res 2020; 12 : 8801-8811 The Anti-Apoptotic Role of EBV-LMP1 in Lymphoma Cells.
Application : WB
Reactivity : Human
Zheng H et al. J Dent Res 2019; : 2.20345E+13 Mdm2 Promotes Odontoblast-like Differentiation by Ubiquitinating Dlx3 and p53.
Application : ICC/IF
Soyoung I. et al. Int J Clin Exp Pathol 2018; 11 (4) : 2246-2255 Application of adipocyte-related antibodies in undifferentiated sarcomas to identify dedifferentiated liposarcomas based on histological and clinical analysis.
Application : IHC-P
Reactivity : Human
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Submit a Reference

REVIEW

MDM2 antibody Cat. No. GTX100531

Rating
( Average 5 based on 1 users reviews)
Application
Western Blot(WB)
( Average 5 based on 1 users reviews)
Anti-MDM2 antibody used in Western Blot (WB). GTX100531
Date : Anonymous submitted on 23-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Sample : MCF7 cells
Amount used : 20μg
Blocking : 5% skim milk in TBS-T, RT, 0.5Hr
Primary Antibody : 1:3000, 4°C, 14Hr
Submit a Review
Application Reference
HC Cheng et al. Cell Mol Life Sci 2023; 80 (11):338 Loss?of?fragile?WWOX?gene?leads?to?senescence?escape?and?genome?instability
Tateishi K et al. Acta Neuropathol Commun 2023; 11 (1):186 Genetic alterations that deregulate RB and PDGFRA signaling pathways drive tumor progression in IDH2-mutant astrocytoma.
Zeng M et al. Cancer Manag Res 2020; 12:8801-8811 The Anti-Apoptotic Role of EBV-LMP1 in Lymphoma Cells.
Application : WB
Reactivity : Human
Zheng H et al. J Dent Res 2019;:2.20345E+13 Mdm2 Promotes Odontoblast-like Differentiation by Ubiquitinating Dlx3 and p53.
Application : ICC/IF
Soyoung I. et al. Int J Clin Exp Pathol 2018; 11 (4):2246-2255 Application of adipocyte-related antibodies in undifferentiated sarcomas to identify dedifferentiated liposarcomas based on histological and clinical analysis.
Application : IHC-P
Reactivity : Human
Tseng CW et al. Cancer Res 2018; (Epub) Transketolase regulates the metabolic switch to control breast cancer cell metastasis via the alpha-ketoglutarate signaling pathway.
Application : WB
Reactivity : Human
Briest F et al. Neuroendocrinology 2017; (Epub) Mechanisms of targeting the MDM2-p53-FOXM1 axis in well-differentiated intestinal neuroendocrine tumors.
Application : WB
Reactivity : Human
Liu Y et al. J Immunol Res 2015; 2015:963568 Autoantibody to MDM2: A Potential Serological Marker of Systemic Lupus Erythematosus.
Application : WB
Reactivity : Human
Chai YR et al. Scand J Immunol 2014; Autoantibodies Response to MDM2 and p53 in the Immunodiagnosis of Esophageal Squamous Cell Carcinoma.
Application : WB
Reactivity : Human
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169