Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ME1 antibody (GTX104122) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using ME1(GTX104122) antibody at 1:200 dilution.

Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ME1 antibody (GTX104122) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Sample (30 μg of whole cell lysate)
A: NIH-3T3
B: JC
C: BCL-1
7.5% SDS PAGE
GTX104122 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunoprecipitation of ME1 protein from HeLa whole cell extracts using 5 μg of ME1 antibody (GTX104122).
Western blot analysis was performed using ME1 antibody (GTX104122).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

ME1 antibody detects ME1 protein at cytosol on human ovarian carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human ovarian carcinoma.
ME1 antibody (GTX104122) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Sample (30 μg of whole cell lysate)
A: PC-12
7.5% SDS PAGE
GTX104122 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.