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MEK2 antibody [5F6]

Overexpression
Anti-MEK2 antibody [5F6] used in IHC (Paraffin sections) (IHC-P). GTX84166
Anti-MEK2 antibody [5F6] used in IHC (Paraffin sections) (IHC-P). GTX84166
Anti-MEK2 antibody [5F6] used in Western Blot (WB). GTX84166
Anti-MEK2 antibody [5F6] used in Western Blot (WB). GTX84166
Anti-MEK2 antibody [5F6] used in IHC (Paraffin sections) (IHC-P). GTX84166
Anti-MEK2 antibody [5F6] used in IHC (Paraffin sections) (IHC-P). GTX84166
Anti-MEK2 antibody [5F6] used in Flow cytometry (FACS). GTX84166

Cat. No. GTX84166

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 5F6

Isotype

 IgG1

Application

 WB, IHC-P, FACS

Reactivity

 Human, Monkey
Package
100 μl ($519)
See all MEK2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000
IHC-P 1:150
FACS 1:100
Not tested in other applications.

Calculated MW

44 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.95 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full-length protein expressed in 293T cell transfected with human MAP2K2 expression vector

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

mitogen-activated protein kinase kinase 2 , CFC4 , MAPKK2 , MEK2 , MKK2 , PRKMK2

Cellular Localization

Cytoplasm,Membrane

Background

Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates the ERK1 and ERK2 MAP kinases.

Database

Research Area

DATA IMAGES

Anti-MEK2 antibody [5F6] used in IHC (Paraffin sections) (IHC-P). GTX84166

GTX84166 IHC-P Image

IHC-P analysis of human ovary adenocarcinoma tissue using GTX84166 MEK2 antibody [5F6].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-MEK2 antibody [5F6] used in IHC (Paraffin sections) (IHC-P). GTX84166

GTX84166 IHC-P Image

IHC-P analysis of human lung tissue using GTX84166 MEK2 antibody [5F6].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-MEK2 antibody [5F6] used in Western Blot (WB). GTX84166

GTX84166 WB Image

Overexpression

WB analysis of HEK293T cells transfected with MEK2 plasmid (Right) or empty vector (Left) for 48 hrs using GTX84166 MEK2 antibody [5F6].
Loading : 5 ug per lane

Anti-MEK2 antibody [5F6] used in Western Blot (WB). GTX84166

GTX84166 WB Image

WB analysis of various cell lines using GTX84166 MEK2 antibody [5F6].
Loading : 10 ug per lane
Dilution : 1:200

Anti-MEK2 antibody [5F6] used in IHC (Paraffin sections) (IHC-P). GTX84166

GTX84166 IHC-P Image

IHC-P analysis of human breast adenocarcinoma tissue using GTX84166 MEK2 antibody [5F6].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-MEK2 antibody [5F6] used in IHC (Paraffin sections) (IHC-P). GTX84166

GTX84166 IHC-P Image

IHC-P analysis of human pancreas tissue using GTX84166 MEK2 antibody [5F6].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-MEK2 antibody [5F6] used in Flow cytometry (FACS). GTX84166

GTX84166 FACS Image

FACS analysis of HEK293T cells transfected with either MEK2 plasmid(Red) or empty vector control plasmid(Blue) using GTX84166 MEK2 antibody [5F6].

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 519