APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:500-1:1000 |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
0.05% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Synthetic phosphopeptide derived from human MEK2 around the phosphorylation site of Threonine 394.
Purification
Purified by antigen-affinity chromatography
From serum
Purity
> 95% (by SDS-PAGE)
Conjugation
Unconjugated
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
mitogen-activated protein kinase kinase 2 , CFC4 , MAPKK2 , MEK2 , MKK2 , PRKMK2
Cellular Localization
Cytoplasm,Membrane
Background
The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is known to play a critical role in mitogen growth factor signal transduction. It phosphorylates and thus activates MAPK1/ERK2 and MAPK2/ERK3. The activation of this kinase itself is dependent on the Ser/Thr phosphorylation by MAP kinase kinase kinases. Mutations in this gene cause cardiofaciocutaneous syndrome (CFC syndrome), a disease characterized by heart defects, cognitive disability, and distinctive facial features similar to those found in Noonan syndrome. The inhibition or degradation of this kinase is also found to be involved in the pathogenesis of Yersinia and anthrax. A pseudogene, which is located on chromosome 7, has been identified for this gene. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
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GTX66729 WB Image
WB analysis of various samples using GTX66729 MEK2 (phospho Thr394) antibody. Lane1 : HEK293T whole cell lysate(40ug)
Lane2 : HEK293T treated with UV for 5 minutes then repair for 16 hours whole cell lysate(40ug)
Lane3 : The Kidney tissue lysate of Rat(40ug)
Lane4 : The Kidney tissue lysate of Mouse(40ug) Dilution : 1:2000
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REFERENCE
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REVIEW
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