Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with MIF antibody [N1C3] (GTX101162) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with MIF antibody [N1C3] (GTX101162) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

MIF antibody [N1C3] detects MIF protein at cytoplasm in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
MIF antibody [N1C3] (GTX101162) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Immunofluorescence analysis of methanol-fixed HeLa, using MIF(GTX101162) antibody at 1:200 dilution.

MIF antibody [N1C3] detects MIF protein at cytoplasm in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
MIF antibody [N1C3] (GTX101162) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

ELISA detection of MIF using GTX101162 for capture at a concentration of 5 μg/mL and GTX89535 for detection at a concentration of 1.5 μg/mL.

Various tissue extracts (50 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with MIF antibody [N1C3] (GTX101162) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Sample (30 μg of whole cell lysate)
A: Molt-4 (GTX27912)
15% SDS PAGE
GTX101162 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.