Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using MMACHC(GTX106822) antibody at 1:100 dilution.

Various tissue extracts (50 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with MMACHC antibody [N1C2] (GTX106822) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunohistochemical analysis of paraffin-embedded human hepatoma, using MMACHC(GTX106822) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with MMACHC antibody [N1C2] (GTX106822) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Immunohistochemical analysis of paraffin-embedded C2C12 xenograft, using MMACHC(GTX106822) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min