Non-transfected (–) and transfected (+) U87-MG whole cell extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MMP2 antibody [HL1207] (GTX636524) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

U87-MG whole cell extract and conditioned medium (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MMP2 antibody [HL1207] (GTX636524) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Mouse tissue extracts (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with MMP2 antibody [HL1207] (GTX636524) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

MMP2 antibody [HL1207] detects MMP2 protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse tissues.
MMP2 stained by MMP2 antibody [HL1207] (GTX636524) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min