Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with MMP3 antibody [N3C1], Internal (GTX100723) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Immunohistochemical analysis of paraffin-embedded A549 xenograft, using MMP3(GTX100723) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with MMP3 antibody [N3C1], Internal (GTX100723) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Immunofluorescence analysis of methanol-fixed HeLa, using MMP3(GTX100723) antibody at 1:200 dilution.

The data was published in the journal Nat Commun in 2017.PMID: 28489070