Sample (50 ug of whole cell lysate)
A: mouse brain
12% SDS PAGE
GTX114731 diluted at 1:1000

MRPL12 antibody [N1C3] detects MRPL12 protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37ºC for 30 min.
Green: MRPL12 protein stained by MRPL12 antibody [N1C3] (GTX114731) diluted at 1:500.
Blue: Hoechst 33342 staining.

Immunohistochemical analysis of paraffin-embedded human breast cancer, using MRPL12(GTX114731) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with MRPL12 antibody [N1C3] (GTX114731) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.