Various tissue extracts (50 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with MUC2 antibody [HL1724] (GTX637372) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

MUC2 antibody [HL1724] detects MUC2 protein by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: MUC2 stained by MUC2 antibody [HL1724] (GTX637372) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10μm.

MUC2 antibody [HL1724] detects secreted MUC2 protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat colon.
MUC2 stained by MUC2 antibody [HL1724] (GTX637372) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

MUC2 antibody [HL1724] detects MUC2 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
MUC2 stained by MUC2 antibody [HL1724] (GTX637372) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min