A431 whole cell and membrane extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with MUL1 antibody (GTX112673) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

MUL1 antibody detects MUL1 protein at cytosol on U87 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded U87 xenograft.
MUL1 antibody (GTX112673) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

MUL1 antibody detects MUL1 protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: MUL1 stained by MUL1 antibody (GTX112673) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar= 10μm.

Drosophila tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with MUL1 antibody (GTX112673) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.