Immunofluorescence of human HeLa cells stained with PI (Red) and monoclonal anti-Maspin antibody (1:500) with Alexa 488 (Green).

Cell lysates of HeLa(20 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Maspin (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.