Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Matriptase / ST14 antibody [M24] (GTX02803) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate.