Indirect ELISA analysis was performed by coating the plate with recombinant Multiple Tags Positive Control ( 6xHis-MBP-DDDDK-V5-HSV-HA-T7-Myc-S) (GTX130342-pro) (57.14-0.89 nM). Coated protein was probed with DDDDK tag antibody [M2-RB] (GTX640415) (1 μg/mL). Mouse Anti-Rabbit IgG (Heavy chain) antibody [GT881] (HRP) (GTX628140-01) (1:10000) was used to detect the bound primary antibody.

Mouse anti-Rabbit IgG Heavy chain [GT881] antibody detects rabbit heavy chain by Western blot analysis.
A. 2 μg denatured rabbit IgG
10 % SDS-PAGE
Mouse anti-Rabbit IgG Heavy chain [GT881] antibody (GTX628140-01) dilution: 1:10000