Indirect ELISA analysis was performed by coating the plate with recombinant Multiple Tags Positive Control ( 6xHis-MBP-DDDDK-V5-HSV-HA-T7-Myc-S) (GTX130342-pro) (57.14-0.89 nM). Coated protein was probed with DDDDK tag antibody [M2-RB] (GTX640415) (1 μg/mL). Mouse Anti-Rabbit IgG (Light chain) antibody [GT1779] (HRP) (GTX628142-01) (1:10000) was used to detect the bound primary antibody.

Denatured rabbit IgG (8 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Mouse anti-Rabbit IgG Light chain (GTX628142) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.