GTX65629-pro GITRL protein induced GITR-mediated NF-κB activation. HEK293 cells were transiently transfected with 20ng of a (NF-κB)-Luc reporter, 2ng of a Renilla luciferase, and with 20ng of a GITR full length encoding construct. At 24hr after transfection, the indicated amounts of GITRL protein or a DDDDK control protein were treated for 4hr and followed by the measurement of dual luciferase activities.