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United States (US)

Mouse IgG3 isotype control [B10]

Cat. No. GTX35027

Reference 2

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 B10

Isotype

 IgG3

Application

 ICC/IF, FACS, IP, ELISA, in vitro
Package
100 μg ($79)
See all Mouse IgG3 isotype control products

APPLICATION

Application Note

Use at a concentration/dilution equivalent to that of your experimental primary antibody.

Product Note

Unknown

PROPERTIES

Form

Liquid

Buffer

Borate buffered saline

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Negative control for Mouse IgG3 lambda , Mouse IgG3 isotype control , Isotype control for Mouse IgG3 lambda , monoclonal Mouse IgG3 isotype control , Mouse IgG3 negative control , Mouse IgG3 lambda isotype control , Isotype control to Mouse IgG3 lambda

Background

Inclusion of an appropriate isotype control is essential for the proper interpretation of experimental results. Because isotype control antibodies have no relevant specificity, they enable the researcher to distinguish non-specific "background" binding from antigen-specific antibody binding. Non-specific binding of primary antibodies may occur due to either binding of antibody to Fc receptors or non-specific interactions with cellular proteins, lipids, or carbohydrates. Additionally, autofluorescence or endogenous enzyme activity in target cells may give a false positive that could be misinterpreted as antibody binding. These factors vary by target cell type and the isotype of the primary antibody. Therefore, the primary antibody used should be matched to an isotype control of identical species and isotype (including both heavy and light chains).

REFERENCE

Application Reference

Shao-Hsuan Wen et al. Int J Mol Sci 2021; 22 (21) Helicobacter pylori Neutrophil-Activating Protein Directly Interacts with and Activates Toll-like Receptor 2 to Induce the Secretion of Interleukin-8 from Neutrophils and ATRA-Induced Differentiated HL-60 Cells.
Vukcevic M et al. J Cell Sci 2013; 126 (Pt 15) : 3485-92 Gain of function in the immune system caused by a ryanodine receptor 1 mutation.
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Package List Price ($)
$ 79