Non-infected (–) and infected (+) BSC-40 whole cell extracts were separated by 10% SDS-PAGE, and the membrane was blotted with Mpox virus B6R antibody [HL3102] (GTX640548) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.