Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Mucin 4 antibody (GTX135631) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Mucin 4 antibody detects Mucin 4 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human colon.
Mucin 4 stained by Mucin 4 antibody (GTX135631) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min