Indirect ELISA analysis was performed by coating the plate with recombinant Multiple Tags Positive Control ( 6xHis-MBP-DDDDK-V5-HSV-HA-T7-Myc-S) (GTX130342-pro) (57.14-0.89 nM). Coated protein was probed with c-Myc antibody [9E10] (GTX20032) (1 μg/mL). Goat antibody Mouse IgG antibody (HRP) (1:10000) was used to detect the bound primary antibody.

Indirect ELISA analysis was performed by coating the plate with recombinant Multiple Tags Positive Control ( 6xHis-MBP-DDDDK-V5-HSV-HA-T7-Myc-S) (GTX130342-pro) (57.14-0.89 nM). Coated protein was probed with Myc tag antibody [GT0002] (GTX628259) (1 μg/mL). Goat antibody Mouse IgG antibody (HRP) (1:10000) was used to detect the bound primary antibody.

Indirect ELISA analysis was performed by coating the plate with recombinant Multiple Tags Positive Control ( 6xHis-MBP-DDDDK-V5-HSV-HA-T7-Myc-S) (GTX130342-pro) (57.14-0.89 nM). Coated protein was probed with DDDDK tag antibody [HL3005] (GTX640415) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect the bound primary antibody.

Lane1: 1 μg of negative control
Lane2: 1 μg of multiple tags positive control (GTX130342-pro) blots were probed with various GeneTex’s Tag antibodies and then probed with goat polyclonal secondary antibody to rabbit IgG - H&L (HRP) (GTX213110-01) or goat polyclonal secondary antibody to mouse IgG - H&L (HRP) (GTX213111-01).