Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Musashi 1 antibody [N3C3] (GTX101540) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

MSI1 antibody [N3C3] detects MSI1 protein on adult mouse brain by immunohistochemical analysis. Sample: Frozen section of adult mouse brain. MSI1 antibody [N3C3] (GTX101540) diluted at 1:500.

Immunofluorescence analysis of paraformaldehyde-fixed A549, using MSI1(GTX101540) antibody at 1:200 dilution.

Various tissue extracts (50 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Musashi 1 antibody [N3C3] (GTX101540) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Musashi 1 antibody [N3C3] detects Musashi 1 protein by immunohistochemical analysis.
Sample: Frozen sectioned adult mouse retina.
Green: Musashi 1 protein stained by Musashi 1 antibody [N3C3] (GTX101540) diluted at 1:250.
Red: Protein kinase C alpha staining.
Blue: Fluoroshield with DAPI (GTX30920).

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

MSI1 antibody [N3C3] detects MSI1 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: MSI1 protein stained by MSI1 antibody [N3C3] (GTX101540) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.

The data was published in the journal Nat Commun in 2014. PMID: 24722317