Various tissue extracts (50 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Myosin Light Chain 2 (MLC-2v) antibody (GTX100888) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Human tissue extract (30 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Myosin Light Chain 2 antibody (GTX100888) diluted at 1:1000.