WB analysis of various recombinant proteins using GTX22480 Myosin Light Chain 2 (phospho Ser19) antibody.
Lane 1 : Regulatory Light Chain Non-Phospho recombinant protein
Lane 2 : Regulatory Light Chain Phospho recombinant protein
Lane 3 : Smooth Muscle Non-Phospho recombinant protein
Lane 4 : Smooth Muscle Phospho recombinant protein
Laoding : 50 ng
Dilution : 1:1000

IHC-P analysis of mouse heart muscle tissue using GTX22480 Myosin Light Chain 2 (phospho Ser19) antibody.
Dilution : 1:100

ELISA analysis of BSA-conjugated non- and phospho-peptides using serially diluted GTX22480 Myosin Light Chain 2 (phospho Ser19) antibody.
Red : Phosphorylated peptide
Green : Non-phosphorylated peptide
Coating : 0.1 μg

Myosin Light chain (phospho Ser20) antibody (GTX22480) was used at 2.5 μg/ml to detect signal in a variety of tissues including multi-human, multi-brain and multi-cancer slides. This image shows strong staining of both vascular and myometrial smooth muscle cells of the uterus. Tissue was formalin-fixed and paraffin embedded. The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.

Myosin Light chain (phospho Ser20) antibody (GTX22480) was used at a 1:5000 dilution to detect myosin light chain by Western blot. Either 13 or 20 ul of a mouse cardiac myocyte lysate was loaded on a 4-20% Criterion gel for SDS-PAGE. Samples were either mock-treated or CLA-treated, as indicated. After washing, a 1:5,000 dilution of HRP conjugated Gt-a-Rabbit IgG (GTX27090) preceded color development using Amersham's substrate system

Western blot of Myosin Light chain (phospho Ser20) antibody (GTX22480). Lane 1: Regulatory Light Chain Non-Phospho recombinant protein. Lane 2: Regulatory Light Chain Phospho recombinant protein. Lane 3: Smooth Muscle Non-Phospho recombinant protein. Lane 4: Smooth Muscle Phospho recombinant protein. Load: 50 ng per lane. Primary antibody at 1:1,000 overnight at 4ºC. Secondary antibody: Peroxidase rabbit secondary antibody at 1:40,000 for 60 min at RT. Blocking for 30 min at RT. Predicted/Observed size: 20 kDa.

The data was published in the journal Development in 2015.PMID: 25715397

The data was published in the journal J Neurosci in 2017.PMID: 28729440