Wild-type (WT) and N-Cadherin knockout (KO) 293T cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with N-Cadherin antibody [N1N3] (GTX112734) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with N-Cadherin antibody [N1N3] (GTX112734) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

N-Cadherin antibody [N1N3] detects N-Cadherin protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded rat liver.
N-Cadherin stained by N-Cadherin antibody [N1N3] (GTX112734) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

N-Cadherin antibody [N1N3] detects CDH2 protein at membrane on hepatoma by immunohistochemical analysis.
Sample: Paraffin-embedded human hepatoma.
N-Cadherin antibody [N1N3] (GTX112734) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Rat tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with N-cadherin antibody (GTX112734) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

N-Cadherin antibody [N1N3] detects N-Cadherin protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
N-Cadherin stained by N-Cadherin antibody [N1N3] (GTX112734) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

N-Cadherin antibody [N1N3] detects N-Cadherin protein by western blot analysis.
A. 30 μg PC-12 whole cell extract
B. 30 μg Rat2 whole cell extract
5% SDS-PAGE
N-Cadherin antibody [N1N3] (GTX112734) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various tissue extracts (50 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with N-Cadherin antibody [N1N3] (GTX112734) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with N-Cadherin antibody [N1N3] (GTX112734) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

N-Cadherin antibody [N1N3] detects N-Cadherin protein at cell membrane by immunofluorescent analysis.
Sample: NT2D1 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: N-Cadherin stained by N-Cadherin antibody [N1N3] (GTX112734) diluted at 1:500.
Blue: Hoechst 33342 staining.

The data was published in the journal Oncotarget in 2016.PMID: 26840566

The data was published in the journal Oncotarget in 2016.PMID: 26840566