Sample (30 ug of whole cell lysate)
A: A549
B: H1299
C: HCT116
D: MCF-7
7.5% SDS PAGE
GTX115913 diluted at 1:1000

Sample (50 ug of whole cell lysate)
A: mouse muscle
7.5% SDS PAGE
GTX115913 diluted at 1:1000

Immunohistochemical analysis of paraffin-embedded C2C12 xenograft, using NARF(GTX115913) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

NARF antibody detects NARF protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: NARF protein stained by NARF antibody (GTX115913) diluted at 1:500.
Blue: Hoechst 33342 staining.