Immunohistochemical analysis of paraffin-embedded human breast cancer, using NBR1(GTX114539) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Sample (30 μg of whole cell lysate)
A: NIH-3T3
B: JC
7.5% SDS PAGE
GTX114539 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Sample (30 μg of whole cell lysate)
A: Jurkat
7.5% SDS PAGE
GTX114539 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.